Ameliorative Effects of Raffia hookeri Pulp Extract on Cisplatin-induced Brain Damage and Consequent Neurobehavioural Changes in Wistar Rats.

Cisplatin (CIS), a known anticancer drug, has side effects initiated by oxidative damage which hinders its use.Raffia hookeri pulp extract (RHPE), reported to possess antioxidant activity should mitigate cisplatin toxicity. The presentstudy examined the potential of RHPE to reduce brain damage in rats exposed to cisplatin. Forty eight female rats (150 g -220 g) were randomized into four groups (n = 12) viz: Group 1 served as control received distilled water daily, Group 2received 100 mg/kg body weight of RHPE, Group 3 received CIS (7.5 mg/kg body weight, intraperitoneally) as single dose,Group 4 received 100 mg/kg body weight of CIS+RHPE. The RHPE was given orally via gavage for 14 days while the singledose of cisplatin was administered on the eighth day of experiment. Behavioral tests namely: transitions, rearings, groomingsand forelimb grip strength were carried out on 15th day of the experiment after which rats were euthanized followed byhistology and histomorphometry. Cisplatin significantly (p<0.05) reduced the percentage body weight changes, transitions,rearings, groomings and forelimb grip strength compared with the control group, whereas treatment with CIS+RHPEsignificantly (p<0.05) increased these parameters compared with Cisplatin treatment. Cisplatin also caused histologicalalterations of Purkinje neurons, pyramidal neurons of Cornu ammonis3, granule cells and cerebral cortex neurons. Itsignificantly (p<0.05) reduced the diameter of Purkinje (9.1±0.59 µm) compared with control (14.41±0.31 µm) andpyramidal neurons (11.32±0.05 µm) compared with control (17.03±0.54 µm). Rats in the CIS+RHPE had their histologyconsiderably improved compared with those of cisplatin. In conclusion, RHPE reversed the behavioural changes anddemonstrated neuroprotection against CIS-induced behavioural changes and microanatomical alterations of cerebellar,hippocampal and cerebral neurons.