A novel air-assisted liquid-liquid microextraction based on in-situ phase separation for the HPLC determination of bisphenols migration from disposable lunch boxes to contacting water.

In this study, a novel air-assisted liquid-liquid microextraction (AALLME) method was developed for the HPLC determination of bisphenols migration from disposable plastic lunch box to contacting water. The AALLME was carried out in commercially-available medical plastic syringes with low-density extraction solvent (n-octanol), and the reaction of NaHCO3 and HCl was introduced to simultaneously induce the in-situ phase separation and salt effect. After the AALLME processes, the upper organic phase was pushed into the end of syringes (narrow tube) by moving the piston so that it could be readily collected for the HPLC analysis. The factors including the type and volume of extraction solvent, NaHCO3 addition and extraction cycles were optimized. Under the optimal conditions, the limits of detection (LODs) for target bisphenols were evaluated to be 0.2-0.7 μg L-1 , and good linearities with correlation coefficients higher than 0.9955 were obtained. The recoveries for target bisphenols ranged from 80% to 106%, with the relative standard deviations (RSDs) of 4.4-14.1%. The proposed method was successfully applied to investigate the migration of bisphenols from the disposable plastic lunch boxes to the contacting water, revealing that compared to the conventional water-bath heating, the microwave heating could induce the significant migration of bisphenols in a much shorter time.