Antiwrinkle and antimelanogenesis activity of the ethanol extracts of Lespedeza cuneata G. Don for development of the cosmeceutical ingredients.

To develop the ingredient with the cosmeceutical function, the antiwrinkle and antimelanogenesis effects of the ethanol extract of Lespedeza cuneata G. Don were investigated. DPPH radical scavenging activity was significantly increased with the extract of L. cuneata G. Don. Cell viability on CCD986Sk human fibroblast was also increased by the ethanol extract of L. cuneata G. Don. The inhibitory function of the extract of L. cuneata G. Don on collagenase, elastase, and tyrosinase was evaluated. Protein expression level of Claudin-1, Occludin, and ZO-1 was up-regulated in HaCaT human keratinocyte by the extract of L. cuneata G. Don. In addition, the extract of L. cuneata G. Don inhibited melanin synthesis in B16F10 murine melanoma cells by decreasing MITF, TRP1, and TRP2 protein levels and increasing the phosphorylated Erk and Akt. Thus, these findings would be useful for developing the new cosmeceutical formulations based on the extract of L. cuneata G. Don.