We have located links that may give you full text access.
Effects of young extracellular matrix on the biological characteristics of aged tendon stem cells.
Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University 2018 July 32
BACKGROUND: Age-related changes in the properties of tendon stem cells (TSCs) may play a role in the progressive degeneration and increased risk of injury to tendon tissue. Recent reports have demonstrated that a decellularized extracellular matrix (DECM) can provide an appropriate niche to maintain the proliferation and differentiation capacity of adult stem cells.
OBJECTIVES: We investigated the biological effects of DECM obtained from young TSCs on the proliferation, stemness, senescence, and differentiation of the aged TSCs.
MATERIAL AND METHODS: Tendon stem cells were isolated from rat patellar tendons and the DECM was collected. The proliferative capacity, β-galactosidase activity, stem cell marker expression, and tenogenic differentiation potential of TSCs were assessed.
RESULTS: Our results showed that DECM from young TSCs enhanced the proliferation and tenogenic differentiation of aged TSCs. Moreover, the senescence-associated β-galactosidase activity of aged TSCs was decreased by young DECM. After being cultured on the young DECM, the expression of stem cell markers by aged TSCs was more extensive. The young DECM preserved stem cell properties of aged TSCs.
CONCLUSIONS: Taken together, the impaired capacity of aged TSCs can be rejuvenated by exposure to young DECM. The positive results in our study suggest that young TSC-derived DECM may provide a novel approach for the prevention and treatment of age-dependent tendon disorders.
OBJECTIVES: We investigated the biological effects of DECM obtained from young TSCs on the proliferation, stemness, senescence, and differentiation of the aged TSCs.
MATERIAL AND METHODS: Tendon stem cells were isolated from rat patellar tendons and the DECM was collected. The proliferative capacity, β-galactosidase activity, stem cell marker expression, and tenogenic differentiation potential of TSCs were assessed.
RESULTS: Our results showed that DECM from young TSCs enhanced the proliferation and tenogenic differentiation of aged TSCs. Moreover, the senescence-associated β-galactosidase activity of aged TSCs was decreased by young DECM. After being cultured on the young DECM, the expression of stem cell markers by aged TSCs was more extensive. The young DECM preserved stem cell properties of aged TSCs.
CONCLUSIONS: Taken together, the impaired capacity of aged TSCs can be rejuvenated by exposure to young DECM. The positive results in our study suggest that young TSC-derived DECM may provide a novel approach for the prevention and treatment of age-dependent tendon disorders.
Full text links
Related Resources
Trending Papers
Challenges in Septic Shock: From New Hemodynamics to Blood Purification Therapies.Journal of Personalized Medicine 2024 Februrary 4
Molecular Targets of Novel Therapeutics for Diabetic Kidney Disease: A New Era of Nephroprotection.International Journal of Molecular Sciences 2024 April 4
The 'Ten Commandments' for the 2023 European Society of Cardiology guidelines for the management of endocarditis.European Heart Journal 2024 April 18
A Guide to the Use of Vasopressors and Inotropes for Patients in Shock.Journal of Intensive Care Medicine 2024 April 14
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app