Piperine attenuates the cancerous activity response in Neuro-2a cell line.

Interactions of cancer cells with their microenvironment play a significant role in defining the severity of the disease. In search of novel compounds with anti-inflammatory and anticancerous capabilities, the effects of purified compound piperine were investigated in Neuro-2a cell line. The neuronal lineage of Neuro-2a cell line was confirmed by using antibody against β-III tubulin protein. The cells were treated with different concentrations of piperine (μM: 10, 50 and 100) for 48 hrs at 37ºC. A dose of 100 µM was selected that induces a 50% inhibition in the cell growth calculated by MTT and morphometery assays. The result shows that in the presence of piperine neurite outgrowth was decreased in a dose dependent manner. The gene expression of TN-C, TNfnD and TnfnC were significantly reduced whereas the expression intensities of TnfnA1, TnfnA2, CSPGs and Laminin were significantly elevated when compared to their respective untreated controls. Similarly proinflammatory marker COX-2 expression was significantly inhibited in the presence of piperine when compared to untreated controls. This is the first time we have illustrated that irrespective of increased expressions of CSPGs, a significant reduction in Tenascin-C and its TNfnD and TNfnC domains are necessary to inhibit the tumor progression. Taken together, the capabilities of piperine to induce an apoptosis by decreasing the neurite outgrowth, proliferation rate and expression of TN-C and COX-2 in Neuro-2a cell line confirmed for its anticancerous and anti-inflammatory potential.