We have located links that may give you full text access.
[Genetic Parameters of SNP Loci in Next Generation Sequencing Kits and Their Comparison].
Fa Yi Xue za Zhi 2018 June
OBJECTIVES: To calculate genetic parameters of SNP loci in next generation sequencing kits, and to compare them with STR loci for establishing the conversion ratio between SNP and STR system effectiveness.
METHODS: Hardy-Weinberg equilibrium tests were performed in 101 SNP loci of next generation sequencing kits (ForenSeq™ DNA Signature Prep kit and Precision ID Identity Panel kit). The parameters of system effectiveness of SNP loci in the cases of personal identification, trios, duos, and alleged parents were calculated, which were compared with the genetic parameters of STR loci.
RESULTS: Except 2 loci without the data of genotype frequency, other 99 SNP loci conformed to Hardy-Weinberg equilibrium tests ( P >0.05). In ForenSeq™ DNA Signature Prep kit, the CDP of 94 SNP loci was 1-1.152 1×10⁻³⁴, CPEtrio was 1-4.416 9×10⁻⁸, CPEduo was 1-8.483 7×10⁻⁵, and CPEAP was 1-1.222 7×10⁻¹². In Precision ID Identity kit, the CDP was 1-2.052 4×10⁻³³, CPEtrio was 1-8.709 3×10⁻⁸, CPEduo was 1-1.163 8×10⁻⁴, and CPEAP was 1-3.725 7×10⁻¹². In the cases of personal identification, trios, duos and alleged parents, the system effectiveness of 2.85, 4.51, 4.88 and 4.55 SNP loci was equal to that of 1 STR locus, respectively.
CONCLUSIONS: With high system effectiveness of SNP loci, the next generation sequencing kits is suitable for personal identification and paternity testing in forensic science.
METHODS: Hardy-Weinberg equilibrium tests were performed in 101 SNP loci of next generation sequencing kits (ForenSeq™ DNA Signature Prep kit and Precision ID Identity Panel kit). The parameters of system effectiveness of SNP loci in the cases of personal identification, trios, duos, and alleged parents were calculated, which were compared with the genetic parameters of STR loci.
RESULTS: Except 2 loci without the data of genotype frequency, other 99 SNP loci conformed to Hardy-Weinberg equilibrium tests ( P >0.05). In ForenSeq™ DNA Signature Prep kit, the CDP of 94 SNP loci was 1-1.152 1×10⁻³⁴, CPEtrio was 1-4.416 9×10⁻⁸, CPEduo was 1-8.483 7×10⁻⁵, and CPEAP was 1-1.222 7×10⁻¹². In Precision ID Identity kit, the CDP was 1-2.052 4×10⁻³³, CPEtrio was 1-8.709 3×10⁻⁸, CPEduo was 1-1.163 8×10⁻⁴, and CPEAP was 1-3.725 7×10⁻¹². In the cases of personal identification, trios, duos and alleged parents, the system effectiveness of 2.85, 4.51, 4.88 and 4.55 SNP loci was equal to that of 1 STR locus, respectively.
CONCLUSIONS: With high system effectiveness of SNP loci, the next generation sequencing kits is suitable for personal identification and paternity testing in forensic science.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app