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Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides.

Ameneh Khatami, Tara M Randis, Anna Chamby, Thomas A Hooven, Margaret Gegick, Evan Suzman, Brady A'Hearn-Thomas, Andrew P Steenhoff, Adam J Ratner
Open Forum Infectious Diseases 2018 July
Group B Streptococcus (GBS) is a perinatal pathogen and an emerging cause of disease in adults. Culture-independent GBS detection relies on polymerase chain reaction (PCR) of conserved genes, including sip . We demonstrate suboptimal sensitivity of the existing sip PCR strategy and validate an improved method based on consensus sequences from >100 GBS genomes.

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