[Sequential analysis of genome of Thelazia callipaeda ].

OBJECTIVE: To investigate the molecular characteristics of genome sequence of Thelazia callipaeda (T. cp) .

METHODS: The obtained T. cp genome assembling data were annotated by using a combination of ab initio gene by softwares, GeneMark and GeneID, and the homology of the experimentally confirmed genes was predicted by software GeMoMa. The results were integrated by software EVM to predict all genes of genome. The obtained genes were annotated in the common public database and three dedicated databases (CAZyme, TCDB and PHI), respectively.

RESULTS: The Scaffolds and Contigs gene structure of T. cp genome (79.34 Mb) was analyzed, and a total of 6 333 genes were obtained. The sequence search was conducted in the public databases using BLASTx, of which 97.85% of the genes could be annotated. The genes annotated in the NR database were the most (98.69%), and those enriched in the KEGG pathway were the least (50.50%). The functional genes were blasted by KOG database and totally 4 517 genes were found. The three special databases (CAZyme, TCDB and PHI) were used to annotate all the genes, and 136, 139 and 1 498 genes were assigned respectively, and the number of genes in the PHI database was the largest. In the cytochrome proprietary database, 238 cytochrome P450 genes were predicted.

CONCLUSIONS: We have preliminarily revealed the T. cp genome structure characteristics and annotation information, and totally 6 333 genes are obtained.