[Role of Ginkgo biloba extract in regulating 5-hydroxytrytamine and its receptor in heart failure mice].

Objective: Exploring the modulation of periphery and central serotonin (5-hydroxytrytamine, 5-HT) and 5-HT(2A) receptor (5-HT(2A)R) in mice with heart failure during the process of heart failure and after treatment. Methods: Thirty mice were allocated into three groups randomly including normal group (NG), heart failure group (HFG) and treatment group (TG) ( n =10). Mice in the NG were sacrificed after evaluating left ventricular ejection fraction (EF) with echocardiogram. Mice in HFG and TG experienced myocardial infarction surgery to achieve EF≤30%, HFG was treated with saline and TG with Ginkgo biloba pills (GBP), once a day for 4 weeks, then EF was assessed again before sacrifice. Cardiac muscle tissue fibrosis was observed and the severity of fibrosis was assessed by semiquantitative method. All the indexes including BNP, serum 5-HT, 5-HT and 5-HT(2A)R in platelet and brain were detected. The data were analyzed by SPSS 19.0. Results: EF: EF in HFG and TG were much lower than that in NG ( P <0.05), TG was much higher than HFG ( P <0.05). BNP: BNP in HFG and TG were much higher than that in NG ( P <0.05), TG was significantly lower than HFG ( P <0.05). HFG showed ventricular remodeling, increased myocardial fibrosis; while myocardial fibrosis decreased in TG. 5-HT in serum: serum 5-HT in HFG was much higher than NG (212±29 ng/L vs 146±5 ng/L, P <0.05), TG was much lower than HFG (163±8 ng/L vs 212±29 ng/L, P <0.05). 5-HT in platelet: platelet 5-HT in HFG was much lower than NG (165±22 ng/L vs 193±19 ng/L, P <0.05), platelet 5-HT in TC was higher than HFG and NG (224±27 ng/L vs 193±19 ng/L, 165±22 ng/L, P <0.05). 5-HT(2A)R in platelet: platelet 5-HT(2A)R in HFG was much lower than NG ( P <0.05), TG was much higher than NG and HFG ( P <0.05). 5-HT in cortex: cortex 5-HT in HFG and TG were much higher than NG (253±4 ng/L, 231±31 ng/L vs 179±9 ng/L, P <0.05), TG was a little lower than HFG (231±31 ng/L vs 253±4 ng/L, P >0.05). 5-HT(2A)R in cortex: cortex 5-HT(2A)R in HFG and TG were much higher than NG ( P <0.05), TG was much lower than HFG ( P <0.05). 5-HT in hippocampus: hippocampus 5-HT in HFG was much higher than NG (250±7 ng/L vs 179±5 ng/L, P <0.05), TG was much lower than HFG (164±6 ng/L vs 250±7 ng/L, P <0.05). 5-HT(2A)R in hippocampus: hippocampus 5-HT in both HFG and TG were much higher than NG ( P <0.05), there was no significant difference between TG and HFG ( P >0.05). Conclusion: Significant changes of 5-HT and 5-HT(2A)R are found in heart failure mice during the process of heart failure and after treatment, indicating the potential value of 5-HT modulation in the diagnose and treatment of heart failure.