Wet electrospun alginate/gelatin hydrogel nanofibers for 3D cell culture.

Convergence of biological and biofabrication approaches is necessary to progress new biomaterials promoting three-dimensional (3D) cell growth and maturation towards tissue regeneration and integration. Here, we have developed a novel approach to fabricate 3D macroporous, alginate/gelatin hydrogel nanofibers (Alg/GelF-MA), which provide superior cell adhesion, motility, proliferation and maturation. The electrospinning process greatly depends on the ionic strength and viscoelastic behavior of the solution. The polyelectrolyte nature of alginate favors intramolecular bundles over intermolecular entanglement, which hinders its electrospinnability. Electrospinning of alginate was achieved by the aid of a supporting polymer, polyethylene oxide and a surfactant, Pluronic®F127. Furthermore, the Ca2+ -mediated coagulation process of alginate was realized in situ during wet electrospinning, where the rapid physical crosslink-ability of alginate was applied in conjunction with the jet entrance into the wet electrospinning collector, a coagulation bath. Consequently, the rapid formation of Ca2+ -alginate complex stabilized the nanofiber morphology. The low surface tension of the non-solvent ethanol used in the bath prevented fibers from dense packing, thus allowing the generation of 3D macroporous structure favoring cell motility. The subsequent UV-mediated chemical crosslinking further stabilized the gelatin content in the Alg/GelF-MA hydrogel nanofibers. It is demonstrated that the Alg/GelF-MA nanofibers with low cytotoxicity (below 10%) supported an over 8-fold proliferation of mesenchymal stem cells over 5 weeks and supported the maturation of human iPSC-derived ventricular cardiomyocytes, which significantly outperform the cell encapsulated bulk GelF-MA hydrogel. The work provides an insight for rational design and development of 3D cell culture matrix for advancement of stem cell therapy and tissue regeneration.