[Oxytetracycline Wastewater Treatment in Microbial Fuel Cells and the Analysis of Microbial Communities].

Oxytetracycline (OTC) as an important broad spectrum antibiotic has been widely used in animal husbandry. However, the abuse of OTC not only has a direct adverse impact on ecosystems, but also exacerbates the appearance of antibiotic resistance bacteria and antibiotic resistance genes. OTC-containing wastewater was treated using microbial fuel cells (MFCs), and the removal efficiency of OTC in MFCs in different operation periods was investigated. Result showed that the removal efficiency of 10 mg·L-1 OTC by MFCs within 132 h was up to 99.0% after 150 d of operation. The bacterial communities in raw pig mature and anodic biofilms were studied by high-throughput sequencing. This showed that Firmicutes were both dominant on phylum a level; However, compared to the raw pig mature, the abundance of Proteobacteria greatly increased from 2.84% to 8.92%-22.75% in the anodic biofilm. In addition, the abundance of Eubacterium spp. in the anodic biofilm increased obviously from nearly 0.00% to 20.49%-49.00%. It has been reported that some Eubacterium spp. were able to biodegrade oxygen heterocyclic aromatic compounds contained in OTC. Therefore, Eubacterium spp. is suggested as potential functional species in the biodegradation of OTC and/or its metabolites. This work proves the feasibility and effectiveness of removing OTC-containing wastewater by using MFCs.