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[Study on species identification of Cronobacter isolated from infant formula foods and cereal based foods and clinical diarrhea cases in 2012-2014 in China].
Wei Sheng Yan Jiu = Journal of Hygiene Research 2017 September
OBJECTIVE: To develop a Cronobacter spieces identification PCR method based on RNA polymerase beta subunit( rpo B) gene and identify species of Cronobacter isolated from different sources.
METHODS: A total of 7 primer pairs based on the rpo B were design, and 9 standard strains were set as reference strains to identify species of261 strains isolated from infant formula foods, cereal based foods and clinical diarrhea cases in 2012-2014 in China.
RESULTS: The molecular weight of 9 standard strains were as same as expect, 179 of 216 Cronobacter isolates were C. sakazakii, 56 were C. malonaticus, 13 were C. universalis, 11 were C. dublinensis, 2 were C. turicensis, accounted for 68. 58%, 21. 46%, 4. 98%, 4. 21% and 0. 77% of the total isolates, respectively.
CONCLUSION: The Cronobacter species identification PCR system is specific, convenient, sensitive. This system can provide technical support for Cronobacter risk surveillance and control in China.
METHODS: A total of 7 primer pairs based on the rpo B were design, and 9 standard strains were set as reference strains to identify species of261 strains isolated from infant formula foods, cereal based foods and clinical diarrhea cases in 2012-2014 in China.
RESULTS: The molecular weight of 9 standard strains were as same as expect, 179 of 216 Cronobacter isolates were C. sakazakii, 56 were C. malonaticus, 13 were C. universalis, 11 were C. dublinensis, 2 were C. turicensis, accounted for 68. 58%, 21. 46%, 4. 98%, 4. 21% and 0. 77% of the total isolates, respectively.
CONCLUSION: The Cronobacter species identification PCR system is specific, convenient, sensitive. This system can provide technical support for Cronobacter risk surveillance and control in China.
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