[Role of nicotinamide vadenine dinucleotide phosphate oxidase 4 in transforming growth factor-β-induced A549 cell migration].

OBJECTIVE: To explore the role of NADPH oxidase 4( NOX4) in transforming growth factor-β( TGF-β)-induced A549 cells migration.

METHODS: The A549 cells were allocated into five groups: TGF-β( stimulation) group, Normal control group, DPI( NOX4 inhibitor) group, TGF-β + DPI group, and DMSO( solvent control)group. The level of ROS were detected by flow cytometry instrument. The level of NOX4, snail and E-cadherin protein were detected by western blot. Use scratches experiment to express the change of A549 cells migration.

RESULTS: After the quantification by Quantity One software, the expression of NOX4 in TGF-β group is( 1. 80 ± 0. 07), the TGF-β +DPI group is( 0. 49 ± 0. 03)( F = 327. 071, P < 0. 001). The change of EMT related protein: the expression of snail protein in TGF-β group is( 9. 0 ± 0. 6), the TGF-β + DPI group is( 1. 8 ± 0. 3)( F = 119. 097, P < 0. 001), the expression of E-cadherin protein in TGF-β group is( 0. 5 ± 0. 1), the TGF-β + DPI group is( 3. 3 ± 0. 3)( F = 71. 063, P <0. 001). These aboveresult indicate that DPI can inhibit the expression of NOX4 and EMTprogress in A549 cells. Then TGF-β + DPI group compared with the TGF-β group, the scratch healing rate is decreased( F = 33. 899, P < 0. 001). It illustrates that DPI can inhibit migration ability of A549 cells.

CONCLUSION: After the NOX4 was inhibited by DPI, TGF-β-induced migration of A549 cells was inhibited. And this phenomenon is associated with the progress of TGF-β-induced EMT.