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Generation and Characterization of Stable α-Synuclein Oligomers.
Alpha-synuclein oligomers are linked to the pathogenesis of Parkinson's disease and related neurodegenerative diseases. In this chapter, we present a method to generate kinetically stable α-synuclein oligomers by the addition of reactive aldehydes, 4-hydroxy-2-nonenal, and 4-oxo-2-nonenal. We also describe biochemical and immunological techniques to characterize the generated oligomers.
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