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Dual Surface Modification of Genome-Free MS2 Capsids for Delivery Applications.
One of the hallmarks of virus-like particles (VLPs) is the fact that they possess distinguishable interior and exterior surfaces. Taking advantage of our knowledge of the amino acid location from X-ray crystal structures, we have developed a series of synthetic modifications of the MS2 bacteriophage viral capsid, including small molecule and polymer attachment, as well as conjugation with peptides, DNA and other proteins. These constructs have found applications in nanomaterial fabrication and as delivery vehicles with therapeutic potential. Importantly, the dual-modification strategies described herein could be extended to other VLP systems.
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