Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA.

Next-generation sequencing (NGS) is increasingly recognized for its ability to deliver high-resolution and high-throughput HLA genotyping. As a result, there is active interest in applying NGS technologies to perform high volume bone marrow donor recruitment typing. Currently, buccal-based DNA specimens are considered a noninvasive and cost-effective method for registry typing. Here, we describe the feasibility of using long-range PCR and clonal sequencing by Illumina MiSeq to deliver unambiguous HLA typing on buccal-based donor recruitment samples.