Add like
Add dislike
Add to saved papers

Quantitation of isoprenoids for natural rubber biosynthesis in natural rubber latex by liquid chromatography with tandem mass spectrometry.

The natural rubber molecule is one of the end products of isoprenoids metabolism in the plant. Dimethylallyl diphosphate (DMAPP) and farnesyl pyrophosphate (FPP) are two typical isoprenoids which control the rate of biosynthesis and the molecular weight of natural rubber. A rapid, nonradioactive method for quantitation of DMAPP and FPP in natural rubber latex by liquid chromatography tandem with mass spectrometry (LC-MS/MS) was reported. DMAPP and FPP were determined in the multiple reaction monitoring mode(MRM)followed by separation with a silica-based C18 column. The external standard quantitative method was established, and the results showed limits of quantitation (LOQs) were 28 ng/ml and 33 ng/ml for DMAPP and FPP, respectively. The concentrations were detected 70-96 ng/ml and 242-375 ng/ml for these two isoprenoids in natural rubber latex. Recoveries of the method were in the range 81-93%. Daytime comparison experiments found that FPP had better stability than DMAPP.

Full text links

We have located links that may give you full text access.
Can't access the paper?
Try logging in through your university/institutional subscription. For a smoother one-click institutional access experience, please use our mobile app.

For the best experience, use the Read mobile app

Mobile app image

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app

All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

By using this service, you agree to our terms of use and privacy policy.

Your Privacy Choices Toggle icon

You can now claim free CME credits for this literature searchClaim now

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app