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Comparative Study
Journal Article
Comparative diagnostics of allergy using quantitative immuno-PCR and ELISA.
Bioanalysis 2018 May 2
AIM: Estimation of specific IgE is essential for the prevention of allergy progression. Quantitative immuno-PCR (qiPCR) can increase the sensitivity of IgE detection. We aimed to develop qiPCR and compare it to the conventional ELISA in identification of IgE to Alt a 1 and Fel d 1 allergens.
RESULTS: Single stranded 60-mer DNA conjugated to streptavidin was used to detect antigen-IgE-biotin complex by qiPCR. In semi-logarithmic scale qiPCR data were linear in a full range of serum dilutions resulting in three- to ten-times higher sensitivity of qiPCR in comparison with ELISA in IgE estimation in low titer sera.
CONCLUSION: Higher sensitivity of qiPCR in identification of low titer IgE is a result of a higher linearity of qiPCR data.
RESULTS: Single stranded 60-mer DNA conjugated to streptavidin was used to detect antigen-IgE-biotin complex by qiPCR. In semi-logarithmic scale qiPCR data were linear in a full range of serum dilutions resulting in three- to ten-times higher sensitivity of qiPCR in comparison with ELISA in IgE estimation in low titer sera.
CONCLUSION: Higher sensitivity of qiPCR in identification of low titer IgE is a result of a higher linearity of qiPCR data.
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