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Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems.
Cold Spring Harbor Protocols 2018 May 17
The Xenopus extract system has been used extensively as a simple, quick, and robust method for assessing the stability of proteins against proteasomal degradation. In this protocol, methods are provided for assessing the half-life of in vitro translated radiolabeled proteins using Xenopus egg or embryo extracts.
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