Patient-Derived iPSC-Hypothamic Neurons: The Ultimate Protocol.

Several methods for making hypothalamic-like neurons (iHTNs) from human or mouse induced pluripotent stem cells (iPSCs) with varying efficiencies exist. In this issue of Cell Stem Cell, Rajamani et al. (2018) devise a new, efficient method to differentiate iPSCs from super-obese and normal donors into iHTNS and investigate their different molecular profiles and hormone responses.