We have located links that may give you full text access.
Mouse Embryo Cryopreservation by High-Osmolality Vitrification.
Cold Spring Harbor Protocols 2018 May 2
In conventional vitrification methods, the embryos are vitrified under considerable supercooling (i.e., under nonequilibrium conditions). This protocol is a refinement of newer equilibrium vitrification methods. The equilibrium vitrification solution contains a high concentration of cryoprotectants that increase its osmolality and allows vitrified embryos to survive not only during storage in liquid nitrogen (LN2 ) at -196°C but also during temporary holding at -80°C (for at least 5 mo). This high-osmolality vitrification (HOV) method is as simple as conventional vitrification and provides essentially the same high postwarming survival rate. It has several advantages over conventional vitrification: (1) Cryopreserved embryos are less likely to be damaged during handling for warming; (2) samples can be temporarily evacuated to a -80°C freezer and can be successfully recovered after 5 mo at -80°C; (3) samples can be arranged and sorted at -80°C; and (4) vitrified embryos can be transported using dry ice. Also included here is an alternative protocol that describes the use of straws instead of cryotubes.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app