Overexpression of miR-1283 inhibits cell proliferation and invasion of glioma cells by targeting ATF4.

It is well known that activating transcription factor 4 (ATF4) expression is closely associated with progression of many cancers. And we found that miR-1283 could directly target the ATF4. However, the precise mechanisms of miR-1283 in glioma have not been well clarified. Our study aimed to explore the interaction between ATF4 and miR-1283 in glioma. In this study, we found that the level of miR-1283 was dramatically decreased in glioma tissues and cell lines, and the expression of ATF4 was significantly increased. And the low level of miR-1283 was closely associated with high expression of ATF4 in glioma tissues. Moreover, introduction of miR-1283 significantly inhibited proliferation and invasion of glioma cells. However, knockdown of miR-1283 promoted the proliferation and invasion in glioma cells. Bioinformatics analysis predicted that the ATF4 was a potential target gene of miR-1283. Luciferase reporter assay demonstrated that miR-1283 could directly target ATF4. In addition, knockdown of ATF4 had the similar effects with miR-1283 overexpression on glioma cells. Up-regulation of ATF4 in glioma cells partially reversed the inhibitory effects of miR-1283 mimic. Overexpression of miR1283 inhibited cell proliferation and invasion of glioma cells by directly down-regulating ATF4 expression.