POLYMORPHISM PREVALENCE OF TLR 9 TYPE GENE IN PATIENTS WITH CHRONIC FORMS OF EPSTEIN-BARR VIRUS-INFECTION.

The aim of the study - to determine the TLR-914 T/C gene polymorphism in patients with CVEB caused by Epstein-Barr virus. Research for the determination of polymorphism - 1486 T/C TLR-9 gene was performed in 44 patients with chronic forms of the VEB infection. DNA isolation from the specimens was performed using a kit for DNA extraction by the Miniprep Company (Siles M, Russia), using the sorption assay technique of the sorbent. Polymorphic region -1486 T/C, rs187084 of the TLR9 gene was studied by real-time PCR amplification by determining the length of the restriction fragment-PCR using restriction enzymes Ncol and oligonucleotide primers. The analysis of the results of the 1486 T/C polymorphism of the TLR-9 gene revealed three main genotypes - TT, TC, CC. Investigation of the frequency of occurrence of individual genotypes revealed the dominance of the genotype TC, compared with the homozygous genotypes of TT and CC. The study of frequency distribution of the TLR-1486 T/C gene polymorphism for different genotypes demonstrated the specificity of changes in the TC genotype in patients with HEBV and the absence of such for the genotypes of TT and CC.