Combining Factors That Individually Enhance Memory in Lymnaea.

When applied individually, thermal stress (1 hour at 30 °C) and (-)epicatechin (a flavonol found in green tea, e.g.) each enhance long-term memory formation following operant conditioning of Lymnaea aerial respiratory behavior. Snails demonstrate enhanced long-term memory formation when trained in epicatechin-treated pond water or when placed in 30 °C pond water for 1 hour, 1 hour prior to training in pond water. We ask here whether the combined application of epicatechin + thermal stress enhances long-term memory retention length beyond the maximal lengths of the individual factors alone. We report that the applied combination of epicatechin + thermal stress has a synergistic memory-enhancing effect; that is, when the two are applied in combination, memory persists longer than when either is applied alone. We then ask whether quercetin, a heat shock protein blocker, will affect the memory enhancement produced by the combined treatment of thermal stress and epicatechin. We report that quercetin does not decrease the memory enhancement of epicatechin, but it does decrease the memory enhancement by thermal stress; and it also decreases the memory persistence of snails exposed to both treatments in combination.