Deciphering the role of substrate stiffness in enhancing the internalization efficiency of plasmid DNA in stem cells using lipid-based nanocarriers.

This study investigates the role of substrate stiffness in the non-viral transfection of human adipose-derived stem cells (hASCs) with the aim to maximize the hASC expression of vascular endothelial growth factor (VEGF). The results confirm the direct effect of substrate stiffness in regulating cytoskeletal remodeling and corresponding plasmid internalization.