Persistent High Levels of miR-502-5p Are Associated with Poor Neurologic Outcome in Patients with Aneurysmal Subarachnoid Hemorrhage.

OBJECTIVE: To investigate the temporal changes in miR-502-5p expression after aneurysmal subarachnoid hemorrhage (aSAH) and to find the time to peak level.

METHODS: We collected serum from patients with aSAH (n = 129) at various time points (1, 3, 7, and 14 days postevent) and healthy controls (n = 40) at the Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College, from May 1, 2015 to January 31, 2016. We measured expression levels of miR-502-5p by polymerase chain reaction. We used the 2-ΔCt method and calculated correlations among variables using Spearman rank correlation coefficient analysis. We used receiver operating characteristic curves to identify optimal levels of miR-502-5p for aSAH and multivariate logistic regression to analyze risk factors on the modified Rankin scale. We measured miR-502-5p expression at all 4 time points post-aSAH.

RESULTS: Levels rose moderately from day 1 to day 7, with a substantial decrease from day 7 to day 14. The peak was at day 7. Multivariate logistic regression revealed that higher miR-502-5p levels at 7 days were associated with a significantly high risk for poor outcome post-aSAH.

CONCLUSIONS: Our data suggest that persistent elevated levels of miR-502-5p participate in the development of aSAH and may help physicians to adjust therapy for aSAH.