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One-step isothermal detection of multiple KRAS mutations by forming SNP specific hairpins on a gold nanoshell.
Analyst 2018 July 24
We developed a one-step isothermal method for typing multiple KRAS mutations using a designed set of primers to form a hairpin on a gold nanoshell upon being ligated by a SNP specific DNA ligase after binding of targets. As a result, we could detect as low as 20 attomoles of KRAS mutations within 1 h.
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