Canine Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Characterization.

Adult multipotent stromal cells (MSCs) are increasingly popular for direct therapeutic applications and bioengineering. Canine patients constitute a major component of veterinary practice, and the dog is an established preclinical animal model for numerous traumatic, degenerative, and disease conditions. Current information supports the presence and relative abundance of adipose tissue-derived multipotent stromal cells (ASCs) in various canine adipose tissue depots. Refined isolation and characterization techniques contribute to collective knowledge of ASC phenotypes and subpopulations for customized, targeted applications. Continued efforts to augment understanding of canine ASCs is critical to progressive treatment advances and high-impact study outcomes. This chapter contains a description of techniques to isolate and characterize canine ASCs.