We have located links that may give you full text access.
Influence of naringenin on the biofilm formation of Streptococcus mutans.
Journal of Dentistry 2018 September
OBJECTIVES: To evaluate the effect of naringenin on the biofilm formation of Streptococcus mutans (S. mutans), and to investigate its mechanisms of action and biological toxicity.
METHODS: Minimum inhibitory concentrations, growth curves, and biofilm inhibition rates of naringenin were determined to assess its antimicrobial effect on S. mutans. The morphology of S. mutans and the structure of biofilm were observed by FESEM and CLSM. Bacterial aggregation, bacterial surface hydrophobicity, and real-time PCR for gtfB, gtfC, comD, comE, and luxS mRNA expression were assessed to preliminarily investigate the mechanisms of action. MTT test using human dental pulp cells (HDPCs) was also performed to investigate cytotoxicity.
RESULTS: The S.mutans growth curves, FESEM, CLSM showed that both 100 and 200 μg/mL of naringenin obviously inhibited S. mutans growth and biofilm formation, increased S. mutans surface hydrophobicity, reduced bacterial aggregation, and downregulated the mRNA expression of gtfB, gtfC, comD, comE, and luxS. However, naringenin at 200 μg/mL slightly decreased the growths of HDPCs compared with 100 μg/mL.
CONCLUSION: Naringenin at 100 and 200 μg/mL suppressed the second (bacterial adhesion) and third stages (biofilm maturation) of S. mutans biofilm formation.
CLINICAL SIGNIFICANCE: Naringenin is promising for dental clinic promotion to prevent the biofilm formation of S. mutans, serving as a safe anti-caries agent at an appropriate concentration.
METHODS: Minimum inhibitory concentrations, growth curves, and biofilm inhibition rates of naringenin were determined to assess its antimicrobial effect on S. mutans. The morphology of S. mutans and the structure of biofilm were observed by FESEM and CLSM. Bacterial aggregation, bacterial surface hydrophobicity, and real-time PCR for gtfB, gtfC, comD, comE, and luxS mRNA expression were assessed to preliminarily investigate the mechanisms of action. MTT test using human dental pulp cells (HDPCs) was also performed to investigate cytotoxicity.
RESULTS: The S.mutans growth curves, FESEM, CLSM showed that both 100 and 200 μg/mL of naringenin obviously inhibited S. mutans growth and biofilm formation, increased S. mutans surface hydrophobicity, reduced bacterial aggregation, and downregulated the mRNA expression of gtfB, gtfC, comD, comE, and luxS. However, naringenin at 200 μg/mL slightly decreased the growths of HDPCs compared with 100 μg/mL.
CONCLUSION: Naringenin at 100 and 200 μg/mL suppressed the second (bacterial adhesion) and third stages (biofilm maturation) of S. mutans biofilm formation.
CLINICAL SIGNIFICANCE: Naringenin is promising for dental clinic promotion to prevent the biofilm formation of S. mutans, serving as a safe anti-caries agent at an appropriate concentration.
Full text links
Related Resources
Trending Papers
Heart failure with preserved ejection fraction: diagnosis, risk assessment, and treatment.Clinical Research in Cardiology : Official Journal of the German Cardiac Society 2024 April 12
Proximal versus distal diuretics in congestive heart failure.Nephrology, Dialysis, Transplantation 2024 Februrary 30
Efficacy and safety of pharmacotherapy in chronic insomnia: A review of clinical guidelines and case reports.Mental Health Clinician 2023 October
World Health Organization and International Consensus Classification of eosinophilic disorders: 2024 update on diagnosis, risk stratification, and management.American Journal of Hematology 2024 March 30
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app