We have located links that may give you full text access.
Uricase based fluorometric determination of uric acid based on the use of graphene quantum dot@silver core-shell nanocomposites.
Mikrochimica Acta 2017 December 19
The authors describe a fluorescent "turn-on" assay for detection of uric acid (UA) based on the use of graphene quantum dots coated with a shell of silver (GQD@Ag). The fluorescence of the GQDs is quenched by the silver shell. However, if the silver shell was removed via etching with H2 O2 (which is produced by uricase catalyzed oxidation of UA), the fluorescence of the GQDs is restored. The resulting increase in fluorescence at 466 nm depends directly on the concentration of H2 O2 , which, in turn, depends on the concentration of UA. The method allows UA to be quantitated with a 2 μM detection limit. It was applied to the analysis of human urine samples and exhibited satisfactory results. The method is cost-effective, sensitive and selective for UA. In our perception, it provides a useful tool in clinical analysis and may be extended to other assays based on the use of oxidases. Graphical abstract Schematic of the reduction of Ag(I) and the growth of a silver shell on the surface of graphene quantum dots (GQDs) to form a GQD@Ag nanocomposite whose fluorescence is quenched. Uricase catalyzes the oxidation of uric acid (UA) to produce allantoin and H2 O2 which etches the silver shell. This results in the release of GQDs and increased fluorescence, allowing quantitative analysis of UA.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app