Genetic Platforms of bla CTX-M in Carbapenemase-Producing Strains of K. pneumoniae Isolated in Chile.

Objective: To elucidate whether the genetic platforms of bla CTX-M contribute to the phenotypes of multi-drug-resistance (MDR) in the first carbapenemase-producing K. pneumoniae strains isolated in Chile. Method: Twenty-two carbapenemase-producing K. pneumoniae strains isolated from different Chilean patients and hospitals were studied. Their genetic relatedness was assessed by PFGE and MLST. The levels of antibiotic resistance were evaluated by determining the minimum inhibitory concentration of various antimicrobials. In addition, several antibiotic resistance genes of clinical relevance in Chile were investigated. The prevalence, allelic variants, and genetic platforms of bla CTX-M were determined by PCR and sequencing. Results: Out of the 22 strains studied, 20 carry KPC, one carries NDM-1, and one carries OXA-370. The PFGE analysis showed three clades with a genetic relatedness >85%, two formed by four strains and one by eight strains. The other strains are not genetically related, and a total of 17 different pulse types were detected. Ten different STs were identified, the main ones being ST258 (five strains) and ST1161 (seven strains). The isolates presented different percentages of resistance, and 82% were resistant to all the β-lactams tested, 91% to ciprofloxacin, 73% to colistin, 59% to gentamicin, 50% to amikacin, and only 9% to tigecycline. All isolates carried bla TEM and bla SHV , whereas 71% carried aac(6 ' )Ib-cr , and 57% one qnr gene ( A, B, C, D , or S ). The bla CTX-M gene was found in 10 of the isolates (4 bla CTX-M-15 and 6 bla CTX-M-2 ). The characterization of the platform, in seven selected strains, revealed that the gene is associated with unusual class 1 integrons and insertion sequences such as IS CR1 , IS ECp1 , and IS 26 . Conclusion: In the first carbapenemase-producing K. pneumoniae strains isolated in Chile the genetic platform of bla CTX-M-2 corresponds to an unusual class 1 integron that can be responsible for the MDR phenotype, whereas the genetic platforms of bla CTX-M-15 are associated with different IS and do not contribute to multi-drug resistance.