[Expression of H19 long non-coding RNA and ZEB1 in the trophoblast of women with spontaneous abortion].

OBJECTIVE: To investigate the expression of H19 long non-coding RNA (lncRNA) and zinc finger E-box-binding protein 1 (ZEB1) in the trophoblast of women with spontaneous abortion.
 Methods: A total of 20 women underwent miscarriage were enrolled as a miscarriage group, while 20 women underwent artificial abortion were enrolled as a control group. Reverse transcriptional fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of H19 lncRNA and miR-200, and Western blot was used to detect the expression of ZEB1.
 Results: Compared to the control group, a tendency of decreased H19 was detected in the miscarriage group, with no significant difference (1.28±0.08 vs 1.66±0.21, P=0.091). There was no significant difference in miR-200 between the 2 groups (0.75±0.35 vs 0.58±0.33, P=0.0.533). The expression of ZEB1 was significantly decreased in the miscarriage group compared to that in the control group (0.22±0.03 vs 0.41±0.04, P=0.0003). The expression of H19 lncRNA and ZEB1 was positively correlated (r=0.529, P=0.0005), and the correlation between H19 lncRNA and miR-200 had no statistical significance (r=0.0293, P=0.858). The correlation between miR-200 and ZEB1 also had no statistical significance (r=-0.132, P=0.416).
 Conclusion: Down-regulation of ZEB1 in the trophoblast might be related to miscarriage. H19 lncRNA may regulate the expression of ZEB1.