Introduction.

The chordate ascidians, the major group of tunicate s, is the best animal group for studying molecular and cellular processes underlying formation of a chordate body plan. For these studies, transgenic technologies are powerful. Transgenesis of ascidians has a long history of more than 20 years, and many practical tips have been accumulated. This book is aimed at summarizing the accumulated techniques in ascidians in addition to concrete research in which transgenic techniques have played pivotal roles. This book is useful for fast assimilation of the techniques and for learning the unique devices developed by the enthusiasm of ascidian researchers.