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Fast and Quantitative Analysis of Ediphenphos Residue in Rice Using Surface-Enhanced Raman Spectroscopy.
Journal of Food Science 2018 April
Detection of residual farm chemicals in agricultural crops is a hot topic in the field of food safety. In this study, ediphenphos residue in rice was detected using surface-enhanced Raman spectroscopy (SERS) on a portable Raman spectrometer. A simple pretreatment method for rice samples was developed, and uniform gold nanorods were used for SERS measurement. Characteristic signals can still be detected when ediphenphos concentration in rice extraction solution was higher than or equal to 0.1 mg/L. Quantitative analysis of ediphenphos was conducted by regression models developed using partial least-squares regression, random forest and kernel principal component analysis, and root-mean-square error of cross validation, coefficient of determination and relative predicted deviation of optimal model were 0.022 mg/L, 0.9967 and 297.45, which indicated the proposed method can predict ediphenphos concentration with high precision. To validate the feasibility of practical application further, rice samples spiked with 10, 5, 1, 0.5, and 0.1 μg/g ediphenphos residue were analyzed using the above method. The predicted recovery was in the range of 93.4% to 102%, and the predicted error was small for residue of each concentration. These results demonstrated that the presented method could be used for accurate and quantitative detection of ediphenphos residue in rice.
PRACTICAL APPLICATION: This study developed a surface-enhanced Raman spectroscopy (SERS) method for detection of ediphenphos in rice coupled with simple extraction protocol and gold nanorods on a portable Raman spectrometer. SERS is a rapid and accurate method which can be applied in agricultural grain safety inspection.
PRACTICAL APPLICATION: This study developed a surface-enhanced Raman spectroscopy (SERS) method for detection of ediphenphos in rice coupled with simple extraction protocol and gold nanorods on a portable Raman spectrometer. SERS is a rapid and accurate method which can be applied in agricultural grain safety inspection.
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