Effect of Qingguang'an II on expressions of OX42 protein and IL-1β mRNA of retinal microglia cells of rats with chronic high intraocular pressure.

The study investigated the effects of Qingguang'an II (a Chinese medicinal preparation) on expressions of OX42 protein and interleukin-1β (IL-1β) mRNA of retinal microglia cells of rats with chronic high intraocular pressure (IOP). SD rats were randomly divided into 6 groups, that were: A: blank group; B: model group; C: Qingguang'an II low dose group; D: Qingguang'an II medium dose group; E: Qingguang'an II high dose group; F: Yimaikang disket (a Chinese medicinal preparation) group. Experimental rats in B, C, D, E, F groups were established the model of chronic high IOP by cauterizing of superficial scleral vein. Tissues of eyes were obtained after intragastric administration for 2 and 4wk. At the time-point of 2wk, OX42 protein and IL-1β mRNA in group B were statistically expressed in higher level comparing with other groups ( P <0.05). Moreover, at the time-point of 4wk, OX42 protein and IL-1β mRNA in groups C, D and E were statistically expressed in lower level comparing with group F ( P <0.05). Besides, OX42 protein and IL-1β mRNA in groups C and D were statistically expressed in higher level comparing with group E ( P <0.05). OX42 protein and IL-1β mRNA in groups C and D were expressed in similar level ( P >0.05). The study indicated that, in the protection of optic nerve of rats with chronic high IOP, the high dose of Qingguang'an II at the time-point of 4wk was the better choice.