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Concentration effect of Quillaja saponin - Co-surfactant mixtures on emulsifying properties.
Journal of Colloid and Interface Science 2018 June 2
HYPOTHESIS: This study examined the emulsifying properties of mixed surfactant systems of Quillaja saponins and food-grade co-surfactants (Na-caseinate, pea protein, rapeseed lecithin, and egg lecithin). We hypothesized to these mixtures may build mixed adsorption layers and thus enhance emulsion stabilization.
EXPERIMENTS: Oil-in-water emulsions (10%, pH 7) were prepared with different concentrations of co-surfactants (0.1-5.0%) alone or mixed with Quillaja saponins (0.05 or 0.5%). Dynamic interfacial tension measurements were performed to characterize the behavior of the surfactants at an oil-water interface.
FINDINGS: Low Quillaja saponin concentrations led to either no changes or substantial increases in particle sizes of protein stabilized emulsions, but d43 -values decreased in lecithin stabilized emulsions at low lecithin concentrations. The dominating effect of Quillaja saponins at high concentrations led to formation of small droplets (d43 ≤2 µm) in all emulsions, except with 2.5% pea proteins. All co-surfactants showed synergistic or additive effects with respect to interfacial tension reductions upon addition of Quillaja saponins (except for egg lecithin with 0.005% Quillaja saponin addition). The results indicated a competing effect for saponin-protein interfaces, but formation of mixed saponin-lecithin interfaces, thus showing that the emulsion stabilization and interfacial properties can be tuned by specific binary surfactant mixtures.
EXPERIMENTS: Oil-in-water emulsions (10%, pH 7) were prepared with different concentrations of co-surfactants (0.1-5.0%) alone or mixed with Quillaja saponins (0.05 or 0.5%). Dynamic interfacial tension measurements were performed to characterize the behavior of the surfactants at an oil-water interface.
FINDINGS: Low Quillaja saponin concentrations led to either no changes or substantial increases in particle sizes of protein stabilized emulsions, but d43 -values decreased in lecithin stabilized emulsions at low lecithin concentrations. The dominating effect of Quillaja saponins at high concentrations led to formation of small droplets (d43 ≤2 µm) in all emulsions, except with 2.5% pea proteins. All co-surfactants showed synergistic or additive effects with respect to interfacial tension reductions upon addition of Quillaja saponins (except for egg lecithin with 0.005% Quillaja saponin addition). The results indicated a competing effect for saponin-protein interfaces, but formation of mixed saponin-lecithin interfaces, thus showing that the emulsion stabilization and interfacial properties can be tuned by specific binary surfactant mixtures.
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