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Extraction of high-quality RNA from Bacillus subtilis with a lysozyme pre-treatment followed by the Trizol method.

Eber Villa-Rodríguez, Cuauhtemoc Ibarra-Gámez, Sergio de Los Santos-Villalobos
Journal of Microbiological Methods 2018 April
A suitable technique (lysozyme combined with Trizol reagent) was developed to improve the RNA yield, purity and integrity from Bacillus subtilis, under different bacterial growth stages. The obtained RNA was intact, having the required characteristics for downstream applications.

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