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[Genotype analysis of Toxoplasma gondii isolated strains from congenital teras and HIV- Toxoplasma co-infected patient in Jiangsu Province].
Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi = Chinese Journal of Schistosomiasis Control 2017 April 26
OBJECTIVE: To identify the genotype of Toxoplasma gondii isolated strains from a congenital teras (KS strain) and an HIV- Toxoplasma co-infected patient in Jiangsu Province.
METHODS: T. gondii DNA of tachyzoites of a isolate from a congenital teras (KS strain) and blood DNA of an HIV- Toxoplasma co-infected patient in Jiangsu Province were extracted, and 11 loci were identified for the genotype by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP).
RESULTS: The complete bands were obtained from the congenital teras (KS strain) and HIV- Toxoplasma co-infected patient in Jiangsu Province, and identified as T. gondii gene type I.
CONCLUSIONS: T. gondii gene type I may be the dominant genotype strain of T. gondii among the women who have the abnormal pregnant outcomes and HIV- Toxoplasma co-infected patients in Jiangsu Province.
METHODS: T. gondii DNA of tachyzoites of a isolate from a congenital teras (KS strain) and blood DNA of an HIV- Toxoplasma co-infected patient in Jiangsu Province were extracted, and 11 loci were identified for the genotype by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP).
RESULTS: The complete bands were obtained from the congenital teras (KS strain) and HIV- Toxoplasma co-infected patient in Jiangsu Province, and identified as T. gondii gene type I.
CONCLUSIONS: T. gondii gene type I may be the dominant genotype strain of T. gondii among the women who have the abnormal pregnant outcomes and HIV- Toxoplasma co-infected patients in Jiangsu Province.
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