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Using Methylation-Specific PCR to Study RB1 Promoter Hypermethylation.

Thaís M McCormick, Maria Da Glória Da C Carvalho
Methods in Molecular Biology 2018
It has increasingly been considered crucial the understanding of DNA methylation of Tumor Suppressor Gene (TSG) promoters, such as that of retinoblastoma 1 gene (RB1), and its role during carcinogenesis. We present a detailed and optimized protocol of the methylation-specific PCR (MSP) technique to study RB1 gene promoter hypermethylation.

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