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Journal Article
Review
Affinity-based separation methods for the study of biological interactions: The case of peroxisome proliferator-activated receptors in drug discovery.
Methods : a Companion to Methods in Enzymology 2018 August 16
Affinity-based methods using immobilized proteins are important approaches for understanding the interactions between small molecules and biological targets. This review is intended to provide an overview of different affinity based separation methods that have been applied to the study of peroxisome proliferator activated receptors (PPARs). The screening of compound to increase screening rates for synthetic and natural ligands of PPAR are reported. Pros and cons of the approaches in ligand discovery initiatives are discussed.
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