Acclimation process of cultivating Chlorella vulgaris in toxic excess sludge extract and its response mechanism.

Chlorella vulgaris was cultivated in the gradually increased proportion of toxic sludge extracts for acclimation, which was obtained from SBR treated synthetic wastewater containing mixed chlorophenols (2,4,6-trichlorophenol and 4-chlorophenol). The growth of C. vulgaris was obviously improved after acclimation with the cell number in the 100% sludge group was 22.75±0.85∗106 cellmL-1 , which was relatively more than the BG11 control group's (20.80±0.35∗106 cellmL-1 ) and apparently over the 100% sludge group (10.78±0.45∗106 cellmL-1 ). Compared with the sludge control sludge group, C. vulgaris in the acclimation group gained 24.1% and 18.2% more relative removal rate about TOC and ecotoxicity, respectively. Proteomics analysis showed that protein spots were more clear and centralized and the clarifications of the different protein spots narrowed from 8 to 5 after acclimation. Proteins related to oxidoreducase activity and energy metabolism were over expressed and C. vulgaris could select the metabolic pathways, especially enhanced pyruvate fermentation, TCA cycle, and glycolysis after acclimation, by over accumulating the corresponding vital enzymes. Conclusively, acclimation was a good method to improve the removal ability and growth of C. vulgaris and algae could acclimatize itself to grow upon the toxic sludge extracts by metabolic selection. We suppose acclimation process was a potential method for algae wastewater treatment and algae cultivation without or reduce dilution.