Add like
Add dislike
Add to saved papers

MicroRNA-146 regulates the inflammatory cytokines expression in vascular endothelial cells during sepsis.

Die Pharmazie 2017 November 2
AIMS: The purpose of this study was to investigate the functional role of microRNA (miR)-146 in sepsis, as well as the underlying mechanism.

METHODS: Human vascular endothelial cell line EA. hy926 cells were treated with lipopolysaccharide (LPS) and/or transfected with miR-146 mimics, inhibitor, and their corresponding controls. Expression of miR-146 was then analyzed after treatment and/or transfection, as well as the expression of inflammatory cytokines, including tumor necrosis factor (TNF)-α, interleukin (IL)-6, intercellular adhesion molecule (ICAM)-1, and E-selectin, and nuclear factor kappa B (NF-κB) binding activity.

RESULTS: The results showed that the expression of miR-146 was significantly downregulated by LPS stimulation compared to the control group (P < 0.05). Also, the expression of miR-146 was remarkably increased by miR-146 mimics but decreased by miR-146 inhibitor following stimulation with LPS (P < 0.05). In addition, the expression levels of TNF-α, IL-6, ICAM-1, and E-selectin were shown to increase following induction by LPS, and further markedly elevated by miR-146 inhibitor (all P < 0.05). However, the expression levels of these inflammatory cytokines were outstandingly decreased by miR-146 mimics (all P < 0.05). Moreover, we observed that the relative NF-κB activity was statistically upregulated by miR-146 inhibitor but downregulated by miR-146 mimics.

CONCLUSIONS: MiR-146 may play an important role in the pathogenesis and development of sepsis by suppressing the expression of inflammatory cytokines.

Full text links

We have located links that may give you full text access.
Can't access the paper?
Try logging in through your university/institutional subscription. For a smoother one-click institutional access experience, please use our mobile app.

For the best experience, use the Read mobile app

Mobile app image

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app

All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

By using this service, you agree to our terms of use and privacy policy.

Your Privacy Choices Toggle icon

You can now claim free CME credits for this literature searchClaim now

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app