Evaluation of Alopecia: A New Processing Technique Combining Vertical and Transverse Sections from a Single Scalp Biopsy Specimen.

Background: Histopathological study of alopecia generally requires both vertical and transverse sections (VS and TS) and this may demand the need for multiple scalp biopsy samples. Here, we suggest a new processing technique which provides both VS and TS from a single biopsy, thus making the interpretation easier.

Materials and Methods: All formalin-fixed scalp biopsy specimens were processed routinely. Nine VS were taken and mounted on 3 glass slides. The block was then melted and the tissue reembedded with the subcutaneous tissue facing downward and the epidermal aspect toward the technician to obtain TSs. Serial 4 μ sections were cut until the tissue was exhausted and every 25th section was mounted on glass slides and stained with H and E.

Results: 100 scalp biopsy specimens with the history of alopecia have been processed using this technique. The technique provides proper visualization of both VS and TS from a single scalp biopsy without compromising the quality. Further, evaluation of the remaining sections with application of special stains can also be possible with this technique which will maximize the information and contribute to the accurate diagnosis of various types of alopecia.

Conclusions: This technique can be used to obtain diagnostically satisfactory VS and TS from a single scalp biopsy specimen by modifying the embedding procedure, thereby avoiding the need for a second biopsy. All types of alopecia can be diagnosed by this effective method.