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Preparation of Antisense Oligonucleotides to Inhibit miRNA Function.

Chengjian Li, Phillip D Zamore
Cold Spring Harbor Protocols 2018 Februrary 2
This protocol is used to design antisense oligonucleotides (ASOs) for specific inhibition of miRNA function in cultured cells. The incorporation of 2'- O -methyl-modifications in ASOs enhances their potency and resistance to degradation, whereas 3'-terminal cholesterol-conjugation facilitates delivery of ASOs into cells.

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