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Development and validation of a functional co-dominant SNP marker for the photoperiod thermo-sensitive genic male sterility pms3 ( p/tms12-1 ) gene in rice.

Breeding Science 2017 December
The fertility conversion of photoperiod thermo-sensitive genic male sterility (P/TGMS) lines in rice is mostly regulated by the P/TGMS genes in different environmental conditions. A point mutation with G-C on the pms3 ( p/tms12-1 ) gene regulates the pollen fertility of Nongken58S and a large amount of Nongken58S-derived lines. In this study, we developed and designed a functional co-dominant marker according to the SNP loci for the pms3 ( p/tms12-1 ) gene. We can differentiate the SNP loci in pms3 ( p/tms12-1 ) gene from another TGMS lines and inbred cultivars using the dpms3-54 marker. The results showed that 376 bp band was detected in the homozygous genotype for pms3 ( p/tms12-1 ), while 359 bp band was detected in the homozygous genotype for non- pms3 ( p/tms12-1 ), two bands with 376 and 359 bp were detected in the heterozygous genotype. The dpms3-54 marker can be used to test the purity of two-line hybrid rice seeds and to divide each of F1 plant into homozygous and heterozygous genotypes at the seedling stage. Thus, this study provide a useful functional marker to detect pms3 ( p/tms12-1 ) gene in different genetic resources and populations, which can be applied to the development and breeding of P/TGMS lines in two-line hybrid rice system with marker-assisted selection.

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