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Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe.

Firoj Ali, Sunil Aute, Sreejesh Sreedharan, H A Anila, Hiwa K Saeed, Carl G Smythe, Jim A Thomas, Amitava Das
Chemical Communications : Chem Comm 2018 Februrary 16
BODIPY derivative, SF-1, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles. SF-1 is compatible with 3D-SIM imaging of individual cells.

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