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A label-free fluorescence assay for hydrogen peroxide and glucose based on the bifunctional MIL-53(Fe) nanozyme.

A label-free nanozyme MIL-53(Fe) with the dual-function of catalyzing and emitting fluorescence was utilized for turn-on fluorescence detection of hydrogen peroxide and glucose. The proposed strategy provides a cost-effective, safe and sensitive method for the design and development of multiple enzyme cascade assays for various biomolecules.

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