A highly selective and sensitive Tb 3+ -acetylacetone photo probe for the assessment of acetazolamide in pharmaceutical and serum samples.

A novel, simple, sensitive and selective spectrofluorimetric method was developed for the determination of Acetazolamide in pharmaceutical tablets and serum samples using photo probe Tb3+ -ACAC. The Acetazolamide can remarkably quench the luminescence intensity of Tb3+ -ACAC complex in DMSO at pH 6.8 and λex  = 350 nm. The quenching of luminescence intensity of Tb3+ -ACAC complex especially the electrical band at λem  = 545 nm is used for the assessment of Acetazolamide in the pharmaceutical tablet and serum samples. The dynamic range found for the determination of Acetazolamide concentration is 4.49 × 10-9 -1.28 × 10-7  mol L-1 , and the limit of detection (LOD) and limit of quantification (LOQ) are (4.0 × 10-9 and 1.21 × 10-8 ) mol L-1 , respectively.