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A senescence-delaying pre-culture medium for transcriptomics of Podospora anserina.
Journal of Microbiological Methods 2018 March
Podospora anserina is an efficient degrader of recalcitrant plant biomass but senesces quickly on most standard pre-culturing media. Among nine pre-culture media, sufficient growth without senescence was only observed on Luria-Bertani medium. The high quality RNA obtained from subsequent transfer cultures was suitable for transcriptomics.
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