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Metabolic Study of Dioecy in Mauritia flexuosa: NMR-based and Chemometric Approaches.
Phytochemical Analysis : PCA 2018 May
INTRODUCTION: Mauritia flexuosa is a dioecious species native to the Amazon, and the gender identification can currently be determined through the differentiation between female and male flowers in the first flowering.
OBJECTIVES: To identify variations in the metabolome profiles of pooled and individual samples of leaves from female and male plants of M. flexuosa in reproductive phase, and the plant in its vegetative phase, using two extraction systems.
MATERIAL AND METHODS: Extractions of leaves were separately conducted using deuterated water and methanol. The extracts were evaluated by NMR spectroscopy and chemometric methods.
RESULTS: Different NMR spectroscopic profiles were observed for females, males, and plants in the vegetative phase. Significant variations were found in the carbohydrate and fatty acid contents for the aqueous and methanolic extracts, respectively. Although principal component analysis (PCA) has not been efficient to distinguish the genders, orthogonal signal correction/partial least squares-discriminant analysis (OPLS-DA) was able to successfully differentiate male and female plants in the reproductive phase, independently on the sampling approach. For plants in the vegetative phase, OPLS-DA method from aqueous extracts of M. flexuosa leaves also distinguished female samples from the others and showed a small overlap between male and young plants, while the classification model was not able to be used for prediction.
CONCLUSIONS: NMR spectroscopy and chemometric-based approach was demonstrated to be useful in the metabolic study of dioecy in M. flexuosa. The extracts of the leaves allowed for differentiation between male and female plants; however, for plants in the vegetative phase, the identification of the gender was not effective. Copyright © 2018 John Wiley & Sons, Ltd.
OBJECTIVES: To identify variations in the metabolome profiles of pooled and individual samples of leaves from female and male plants of M. flexuosa in reproductive phase, and the plant in its vegetative phase, using two extraction systems.
MATERIAL AND METHODS: Extractions of leaves were separately conducted using deuterated water and methanol. The extracts were evaluated by NMR spectroscopy and chemometric methods.
RESULTS: Different NMR spectroscopic profiles were observed for females, males, and plants in the vegetative phase. Significant variations were found in the carbohydrate and fatty acid contents for the aqueous and methanolic extracts, respectively. Although principal component analysis (PCA) has not been efficient to distinguish the genders, orthogonal signal correction/partial least squares-discriminant analysis (OPLS-DA) was able to successfully differentiate male and female plants in the reproductive phase, independently on the sampling approach. For plants in the vegetative phase, OPLS-DA method from aqueous extracts of M. flexuosa leaves also distinguished female samples from the others and showed a small overlap between male and young plants, while the classification model was not able to be used for prediction.
CONCLUSIONS: NMR spectroscopy and chemometric-based approach was demonstrated to be useful in the metabolic study of dioecy in M. flexuosa. The extracts of the leaves allowed for differentiation between male and female plants; however, for plants in the vegetative phase, the identification of the gender was not effective. Copyright © 2018 John Wiley & Sons, Ltd.
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